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biomarker 1

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drug sensitivity 1

gene therapy 1

hepatocarcinoma 1

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murine ascites hepatocarcinoma cell line 1

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Serum mitochondrial tsRNA serves as a novel biomarker for hepatocarcinoma diagnosis

Frontiers of Medicine 2022, Volume 16, Issue 2,   Pages 216-226 doi: 10.1007/s11684-022-0920-7

Abstract: Hepatocellular carcinoma (HCC), which makes up the majority of liver cancer, is induced by the infection of hepatitis B/C virus. Biomarkers are needed to facilitate the early detection of HCC, which is often diagnosed too late for effective therapy. The tRNA-derived small RNAs (tsRNAs) play vital roles in tumorigenesis and are stable in circulation. However, the diagnostic values and biological functions of circulating tsRNAs, especially for HCC, are still unknown. In this study, we first utilized RNA sequencing followed by quantitative reverse-transcription PCR to analyze tsRNA signatures in HCC serum. We identified tRF-Gln-TTG-006, which was remarkably upregulated in HCC serum (training cohort: 24 HCC patients vs. 24 healthy controls). In the validation stage, we found that tRF-Gln-TTG-006 signature could distinguish HCC cases from healthy subjects with high sensitivity (80.4%) and specificity (79.4%) even in the early stage (Stage I: sensitivity, 79.0%; specificity, 74.8%; 155 healthy controls vs. 153 HCC patients from two cohorts). Moreover, in vitro studies indicated that circulating tRF-Gln-TTG-006 was released from tumor cells, and its biological function was predicted by bioinformatics assay and validated by colony formation and apoptosis assays. In summary, our study demonstrated that serum tsRNA signature may serve as a novel biomarker of HCC.

Keywords： tsRNA     biomarker     hepatocarcinoma    

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Establishment and drug sensitivity evaluation of murine ascites hepatocarcinoma cell line with high lymphatic

Hongying ZHANG, Jianwu TANG, Wenting ZHU, Chunxiu HU, Guowang XU

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 119-129 doi: 10.1007/s11684-009-0022-9

Abstract: Murine ascites hepatocarcinoma cell strain with low lymphatic metastatic potential (Hca-P) was subcutaneouslyhuman hepatocarcinoma cell lines (SMC7721 and HepG ) to curcumin were studied by use of 3-(4,5-dimethylthiazolCurcumin had significant growth inhibiting effect on both murine ascites and human hepatocarcinoma cellAt concentrations of 30-120 μmol/L, curcumin had more inhibition on murine ascites hepatocarcinoma celllines than on human anchorage-dependent hepatocarcinoma cell lines.

Keywords： murine ascites hepatocarcinoma cell line     metastasis     curcumin     drug sensitivity    

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transporter 2 genes for increased retention of metaiodobenzylguanidine labeled with iodine 131 in malignant hepatocarcinoma

Yanlin Zhao,Xiao Zhong,Xiaohong Ou,Huawei Cai,Xiaoai Wu,Rui Huang

Frontiers of Medicine 2017, Volume 11, Issue 1,   Pages 120-128 doi: 10.1007/s11684-017-0501-3

Abstract: To prolong the retention of 131I-MIBG in target cells, we infected hepatocarcinoma (HepG2

Keywords： norepinephrine transporter     vesicular monoamine transporter 2     -MIBG     gene therapy     lentivirus vector    

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Title Author Date Type Operation

Serum mitochondrial tsRNA serves as a novel biomarker for hepatocarcinoma diagnosis

Journal Article

Establishment and drug sensitivity evaluation of murine ascites hepatocarcinoma cell line with high lymphatic

Hongying ZHANG, Jianwu TANG, Wenting ZHU, Chunxiu HU, Guowang XU

Journal Article

transporter 2 genes for increased retention of metaiodobenzylguanidine labeled with iodine 131 in malignant hepatocarcinoma

Yanlin Zhao,Xiao Zhong,Xiaohong Ou,Huawei Cai,Xiaoai Wu,Rui Huang

Journal Article